Analysis of DNHD1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2220-LGZ)

Name: Analysis of DNHD1 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-2220-LGZ
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Introduction

Official Full Name: dynein heavy chain domain 1
Also known as: DHCD1; CCDC35; DNHD1L; SPGF65; C11orf47
Predicted to enable dynein intermediate chain binding activity; dynein light intermediate chain binding activity; and minus-end-directed microtubule motor activity. Predicted to be involved in cilium movement. Located in extracellular exosome.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.