Analysis of CYP2R1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2401-LGZ)

Name: Analysis of CYP2R1 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-2401-LGZ
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Introduction

Official Full Name: cytochrome P450 family 2 subfamily R member 1
This gene encodes a member of the cytochrome P450 superfamily of enzymes. Cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids, and other lipids. This enzyme is microsomal vitamin D hydroxylase, which converts vitamin D to the active ligand for the vitamin D receptor. Mutations in this gene are associated with selective 25-hydroxyvitamin D deficiency.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.