Analysis of CSRP1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0904-LGZ)

Name: Analysis of CSRP1 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-0904-LGZ
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Introduction

Official Full Name: cysteine and glycine rich protein 1
Also known as: CRP; CRP1; CSRP; CYRP; D1S181E; HEL-141; HEL-S-286
This gene encodes a member of the cysteine-rich protein (CSRP) family. This gene family includes a group of LIM domain proteins, which may be involved in important regulatory processes of development and cell differentiation. The LIM/double zinc finger motif found in this gene product occurs in proteins with key functions in gene regulation, cell growth, and somatic cell differentiation. Alternative spliced transcript variants have been described.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.