Analysis of CSF3R Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0096-LGZ)

Name: Analysis of CSF3R Gene (Mutation) by RT-qPCR
SKU: STEM-MT-0096-LGZ
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Introduction

Also known as: SCN7; CD114; GCSFR
The protein encoded by this gene is the receptor for colony-stimulating factor 3, a cytokine that controls granulocyte production, differentiation and function. The encoded protein is a member of the cytokine receptor family and may also play a role in certain cell surface adhesion or recognition processes. Alternative spliced transcript variants have been described. Mutations in this gene are responsible for Kostmann syndrome, also known as severe congenital neutropenia.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Hereditary neutrophilia, Autosomal recessive severe congenital neutropenia due to CSF3R deficiency, Autosomal recessive severe congenital neutropenia due to CSF3R deficiency

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.