Analysis of Complement C3 (Human) by ELISA (CAT#: STEM-MB-0750-LGZ)

Name: Analysis of Complement C3 (Human) by ELISA
SKU: STEM-MB-0750-LGZ
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Introduction

C3 is the complement component with the highest content in serum, which is mainly synthesized by macrophages and liver. Under the action of C3 invertase, C3 is cleaved into C3a and C3b fragments, which play an important role in both the classical and bypass activation pathways of complement.

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Principle Applications Procedure Materials

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.