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Analysis of CKBB (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0109-LGZ)

Introduction

Creatine kinase BB(CK-BB) is a creatine kinase. CK-BB is a homodimer composed of two identical brain CKB subunits. It is a cytoplasmic enzyme involved in cell energy homeostasis. Some parts of CK-BB bind to the cell membrane, ATPase and various intracellular enzymes requiring ATP. In addition, creatine kinase M(CKM) has been identified as an interaction factor for CKB.

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Principle Applications Procedure Materials

Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Immunology/Inflammation, Neurobiology/Neurodegeneration

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Serum, Plasma
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