Analysis of CEBPZ Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1800-LGZ)

Introduction

Official Full Name: CCAAT enhancer binding protein zeta<br />Also known as: CBF; CBF2; NOC1; HSP-CBF<br />This gene belongs to the CBF/Mak21 family. The encoded proteins function in cellular responses to environmental stimuli through a transcriptional process involving heat shock factors, conserved DNA elements (heat shock elements or HSEs), and CCAAT boxes. This protein acts as a DNA-binding transcriptional activator that regulates the heat shock protein 70 (HSP70) promoter in a ccaat-dependent manner. This protein is also involved in cell growth and differentiation, especially hematopoietic differentiation.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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