Analysis of BMAL1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2408-LGZ)

Name: Analysis of BMAL1 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-2408-LGZ
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Introduction

Official Full Name: basic helix-loop-helix ARNT like 1
Also known as: TIC; JAP3; MOP3; ARNTL; PASD3; ARNTL1; BMAL1c; bHLHe5
The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This heterodimer binds to E-box enhancer elements upstream of period genes (PER1, PER2, PER3) and cryptochrome genes (CRY1, CRY2), activating the transcription of these genes. PER and CRY proteins repress their own transcription by interacting with the CLOCK/ARNTL complex in a feedback loop. Defects in this gene have been linked to infertility, problems with gluconeogenesis and lipogenesis, and altered sleep patterns. The protein regulates interferon-stimulated gene expression and is an important factor in viral infections, including COVID-19.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.