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Analysis of Aflatoxigenic Fungal Contamination in Rice by Near Infrared (NIR) Spectroscopy (CAT#: STEM-ST-1519-CJ)

Introduction

Aflatoxin B1 (AFB1) is a naturally occurring mycotoxin that can result in detrimental side effects if consumed by humans or animals. AFB1 is produced by filamentous fungi corresponding to Aspergillus section Flavi particularly Aspergillus flavus. AFB1 is found in a wide range of agricultural products including rice, with greater prevalence in tropical and humid climates. The International Agency for Research on Cancer (IARC) has classified AFB1 as a carcinogen for animals and humans and many countries have strict legal regulations concerning AFB1 contamination in food and agricultural products.

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Principle Applications Procedure Materials Notes

Principle

Near-infrared spectroscopy (NIRS) is a method that uses light between the visible and infrared wavelength ranges (approximately 800 – 2500 nm) and measures the absorption of light as a function of wavelength. The near-infrared light that interacts with the sample has specific wavelengths that are absorbed based on certain chemical bonds (e.g. C-H, O-H, S-H, and N-H) being present in the sample.

Applications

Food Chemistry; Biochemistry; Toxicology

Procedure

1. Prepare samples.
2. Place the sample in the Near Infrared Spectroscopy.
2. Analyze data and identify samples.

Materials

• Sample: Molecules with C-H, O-H, S-H, and/or N-H bonds (Such as: Proteins, Peptide, Water, Alcohol, Fats, Oils, Hydrocarbons); Volatile Organics; Moisture; Fatty Acids; Solids; Carbohydrate; Liquids, etc.
• Equipment: Near Infrared (NIR) Spectroscopy

Notes

1. NIR is non-destructive, and requires little or no sample preparation.
2. NIR reflectance spectra can be used to quickly determine a material’s properties without altering the sample.
3. NIR can depending on wavelength penetrate up to 20 mm of a sample which makes NIR more effective for solid samples.
4. FTIR spectroscopy is, in principle, very similar to Near Infrared (NIR) spectroscopy, but works at longer wavelengths where the chemical information from the samples is more specific.
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