Analysis of Actinobacillus Pleuropneumoniae by Real-Time PCR Method (CAT#: STEM-MB-3541-LGZ)

Introduction

Actinobacillus pleuropneumoniae (APP), previously designated as Haemophilus pleuropneumoniae or H. parahaemolyticus, is the cause of pleuropneumonia of swine. This bacterium is a gram-negative encapsulated coccobacillary rod, which requires nicotinamide adenine dinucleotide (NAD or factor V) for growth.
A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.
Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.