Analysis Biomolecular Interactions of ch-2C5 for S1-RBD Protein by BLI (CAT#: STEM-MB-0171-CJ)

Introduction

The causative agent of COVID-19 is severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a novel strain of human β-coronavirus that belongs to the Coronaviridae family. The genome comprises a plus-sense, single-stranded RNA of 29 kb that encodes four major structural proteins [spike (S), nucleocapsid (N), membrane (M) and envelope (E)] and several non-structural proteins. The S protein, which mediates entry of SARS-CoV-2 into host cells, comprises two subunits (S1 and S2). The S1 subunit contains the N-terminal domain and the receptor-binding domain (RBD); the latter binds to human angiotensin converting enzyme 2 (ACE2) through the receptor-binding motif. S2 promotes fusion between the virus and the host cell membrane. The S protein therefore plays an essential role in virus attachment, receptor binding, membrane fusion, tissue tropism and host range, and it induces the production of neutralizing antibodies, as well as T cell responses.