AMPK Pathway Assay (CAT#: STEM-MB-0358-WXH)

Introduction

AMP-activated protein kinase (AMPK) signaling pathway, a fuel sensor and regulator, promotes ATP-producing and inhibits ATP-consuming pathways in various tissues. AMPK is exists as a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits.
AMPK is a central regulator of cellular energy homeostasis, playing critical roles in regulating growth and reprogramming metabolism as well as in cellular processes including autophagy and cell polarity. The kinase is activated in response to stresses that deplete ATP supplies such as low glucose, hypoxia, ischemia, and heat shock. AMPK activation works through direct phosphorylation of multiple enzymes directly involved in these processes as well as through transcriptional control of metabolism by phosphorylating transcription factors, co-activators, and co-repressors.