IL-33 Detection (CAT#: STEM-MB-0299-WXH)

The trans-model ST2L and receptor accessory protein (AcP) together constitute the membrane surface receptor of IL-33. The receptors of IL-1 molecules are composed of two chains, one of which is responsible for binding to the ligand, and the other is responsible for the activation of the signal to the cell. ST2 is responsible for binding to IL-33, while AcP is the signaling chain. IL-33 binds heterodimers composed of ST2 and AcP on the cell membrane to transmit signals into the cell, recruiting downstream signaling molecules myeloid differentiation factor 88 (MyD88), IL-1 receptor related kinase (IRAK) and tumor necrosis Factor receptor-associated factor 6 (TRAF6) also activates downstream mitogen-activated protein kinase (MAPKK), which activates AP-1 through c-junN-terminal kinase (JNK). TRAF6 can also activate the nuclear factor NF-kB kinase inhibitor complex, leading to the release of NF-kB from the complex, and ultimately lead to the production and function of Th2 cytokines IL-4, IL-5 and IL-13.

IL-33 regulates gene expression and acts as a transcription factor.
IL-33 can polarize naive T cells to produce Th2 cytokines.
IL-33 can cause mast cells and basophils to secrete inflammatory cytokines and chemokines, resulting in NK cells and NKT cells secreting Th1-type cytokines.
IL-33 can enhance the differentiation of M2 macrophages, induce the maturation of dendritic cells, and promote the Th1-type response.

1. Process samples.
2. IL-33 detection (qPCR, Enzyme-linked immunosorbent assay (ELISA), Flow cytometry).
3. Analysis results.

Sample Types-Blood, serum, plasma, cerebrospinal fluid, cell culture supernatant, tissue homogenate, cell culture medium, urine, tumor, etc.