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Display the organizational structure of the auditory cortex in a mouse brain slice by HE staining technology (CAT#: STEM-CT-0004-LJX)

Introduction

Morphological technique is one of the most commonly used neurobiological research techniques. Neural tissue consists of neurons and a variety of glial cells. There are hundreds of nuclear clusters in the brain, and the positions and functions of each cluster are very different. Therefore, morphological technique is of special significance to elucidate the structure and function of the nervous system. To study the histological structure of the central nervous system, the first step is to prepare histological sections. After the section is made, it needs to be dyed so that the tissue structure can be distinguished under the microscope. The prepared mouse tissue sections were dyed to observe the structure, so as to conduct morphological analysis.




Principle

HE staining, hematoxylin-eosin staining, is the most commonly used staining method in morphology. Hematoxylin dye solution is alkaline, which makes chromatin in the nucleus and ribosome in the cytoplasm purplish blue. Eosin is an acidic dye that mainly colors the cytoplasm and extracellular matrix components red. The properties that tend to be colored by alkaline or acidic dyes are called basophilic and acidophilic, respectively; If the affinity with both dyes is not strong, it is called neutral. General tissue changes and tissue products can be shown by this staining method.

Applications

Staining of mouse nerve tissue section samples

Procedure

1.Dewaxing
2.Staining
3.Washing
4.Ethanol hydrochloric acid separation
5.Rinsing
6.Dehydration
7.Restaining
8.Dehydration
9.Transparency
10.Seal

Materials

• Sample Type:
Mouse brain tissue

Notes

Cover glass of different specifications should be selected according to the size of the material before sealing.