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Chiral and structural analysis of biomolecules by ion mobility-mass spectrometry (CAT#: STEM-ST-0095-LJX)

Introduction

Chiral molecules are molecules with a certain configuration or conformation that are different from their mirror images and cannot be overlapped with each other. Cahn et al. proposed to use "chirality" to express the relationship between optically active molecules and stereoscopic images whose mirrors cannot overlap. Chirality is equal to the relationship between left and right hands, which cannot overlap each other. All chiral molecules are optically active, and all molecules of optically active compounds are chiral. Chiral molecules include asymmetric molecules that do not have any symmetry factors and asymmetric molecules that have simple symmetry axes but no other symmetry factors.




Principle

Ion mobility spectrometry–mass spectrometry (IMS-MS) is an analytical chemistry method that separates gas phase ions based on their interaction with a collision gas and their masses. In the first step, the ions are separated according to their mobility through a buffer gas on a millisecond timescale using an ion mobility spectrometer. The separated ions are then introduced into a mass analyzer in a second step where their mass-to-charge ratios can be determined on a microsecond timescale.

Applications

For studying the gas phase ion structure
For detecting the chemical warfare agents and explosives
For the analysis of proteins, peptides, drug-like molecules and nano particles
For monitoring isomeric reaction intermediates and probe their kinetics
For proteomics and pharmaceutical analysis

Procedure

1. Add sample
2. The ions in the sample are separated in the ion mobility spectrometer
3. The separated ions are introduced into the mass analyzer for detection
4. Store the detection results

Materials

• Sample Type:
Biomolecules

Notes

1. Ion mobility spectrometry is also a very fast technique, making it suitable for high-throughput applications. The entire analysis can be completed in just a few minutes.
2. The method is extremely sensitive and able to detect trace amounts of contaminants that other spectrometry methods would miss.
3. The effective separation of analytes achieved with this method makes it widely applicable in the analysis of complex samples such as in proteomics and metabolomics.