Analysis of Measuring Single-Domain Antibody Interactions with Epitopes in Jet Fuel by Microscale Thermophoresis (MST) (CAT#: STEM-MB-2468-LGZ)

Introduction

Microscale Thermophoresis (MST) is the directed movement of particles in a microscopic temperature gradient. Affinity is determined by measuring trace thermal changes resulting from changes in the hydration layer (usually caused by changes in the structure/conformation of biomolecules). Even small changes like protein phosphorylation or small molecules binding to a target can be measured. MST also allows measurement of intermolecular interactions directly in solution without the need for a fixed surface (no fixation required). Microscale Thermophoresis (MST) is a rapid, simple method to detect binding of a single-domain antibody in the presence of jet fuel/water mixes;a complex, harsh environment. This work provides key data to quantifying binding for the development of a sensor for detection of microbial growth in fuel.