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Analysis of Insulin (Human, Non-human primate, Canine) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0369-LGZ)

Introduction

Insulin is a protein hormone secreted by the islet β cells in the pancreas when stimulated by endogenous or exogenous substances such as glucose, lactose, ribose, arginine, and glucagon. Insulin is the only hormone in the body that lowers blood sugar, and at the same time promotes the synthesis of glycogen, fat, and protein. Insulin is an important polypeptide hormone that regulates carbohydrate metabolism. Abnormal level of insulin is associated with diabetes mellitus characterized by chronic hyperglycemia. Therefore, reliable quantification of insulin is important for clinical purpose such as diabetes diagnosis and treatment.




Principle

Detection Technique: MSD Hypersensitive Multi-Factor Electrochemiluminescence
Capture Antibody: Mouse Monoclonal
Detection Antibody: Mouse Monoclonal
Recombinant Standards: Expressed in E. coli

Applications

Metabolic

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Cell Culture Supernatant, P800 Plasma, Serum