Analysis of GAPDH (Human, Non-human primate) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0197-LGZ)

Introduction

GAPDH catalyzes the oxidation of glyceraldehyde 3-phosphate to produce 1, 3-diphosphoglycerate, which is a key enzyme in glycolysis pathway. It is closely related to gluconeogenesis pathway, maintenance of blood glucose concentration in vivo and occurrence of diabetes, and plays an important role in disorders of sugar, lipid and protein metabolism in the body.

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Principle Applications Procedure Materials Notes Related Products

Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Phosphoproteins, Intracellular Signaling

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum

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