Analysis of Diamine oxidase (DAO) Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1459-LGZ)

Introduction

Sensitivity: 7.39 U/L<br />Detection range: 7.39-134.15 U/L<br />Precision: inter-lot difference of 7%, intra-lot difference of 5%<br />Detection equipment: Enzyme-labeled Instrument<br />Detection wavelength: 450-470 nm

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Principle Applications Procedure Materials Notes Related Products

Principle

Diamine oxidase can catalyze amine substances to produce hydrogen peroxide, and hydrogen peroxide can make chromogenic substances produce color. The chromogenic substances have characteristic absorption peaks at 460 nm. The activity of diamine oxidase can be calculated by measuring the change rate of absorbance per unit time.

Applications

It is used to detect the activity of diamine oxidase (DAO) in serum (plasma), urine, animal tissue and cell samples.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: serum (plasma), urine, animal tissue and cell samples

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