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Analysis of COX-2/PTGS2 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0119-LGZ)

Introduction

COX2 is a key enzyme in the process of prostaglandin biosynthesis. It acts as a dioxygenase and a peroxidase, mediates the formation of prostaglandins from arachidonic acid, and plays a special role in the inflammatory response process. By inhibiting COX2 to rapidly reduce inflammatory, pain and febrile responses, long-term use of these drugs reduces fatal blood clot events, as well as the development of colon cancer and Alzheimer's disease.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Immunology/Inflammation

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Serum, Plasma