Analysis of CD1D Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0828-LGZ)

Introduction

Official Full Name: CD1d molecule<br />Also known as: R3; CD1A; R3G1<br />This gene encodes a distinct member of the CD1 family of transmembrane glycoproteins that is structurally related to major histocompatibility complex (MHC) proteins and forms heterodimers with beta-2 microglobulin. The CD1 protein mediates the self or microbial origin of major lipid and glycolipid antigens to T cells. The human genome contains five CD1 family genes, which are arranged on chromosome 1. CD1 family members are thought to differ in their cellular localization and specificity for specific lipid ligands. The protein encoded by this gene localizes to late endosomes and lysosomes via a tyrosine motif in the cytoplasmic tail. Two transcript variants of this gene have been found to encode different isoforms.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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